发明名称 |
N-GLYCOSYLATION OF PEPTIDES AND PROTEINS |
摘要 |
A process for the production of a glycoconjugate by N-glycosylation of a protein or peptide comprising the sequence D/E-X—N—X—S/T, wherein each X is the same or different and is any natural amino acid other than proline, wherein the process comprises reacting the protein or peptide with a polyisoprenyl pyrophosphate of formula (I), or a salt thereof, in the presence of PglB: (I) to produce the glycoconjugate comprising the protein or peptide having a saccharide [SI] linked to the asparagine in the sequence D/E-X—N—X—S/T. Polyisoprenylpyrophosphates used as substrates in the biocatalytic process are also provided, as well as certain glycoconjugates. |
申请公布号 |
EP3057975(A1) |
申请公布日期 |
2016.08.24 |
申请号 |
EP20140787034 |
申请日期 |
2014.10.15 |
申请人 |
OXFORD UNIVERSITY INNOVATION LIMITED;GLYCOVAXYN AG |
发明人 |
DAVIS, BENJAMIN G.;VIJAYAKRISHNAN, BALAKUMAR;LUI, FENG;WACKER, MICHAEL;FARIDMOAYER, AMIRREZA;KOWARIK, MICHAEL THOMAS |
分类号 |
C07H11/04;C07K9/00;C12P19/18 |
主分类号 |
C07H11/04 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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