发明名称 |
ENGINEERED MICROORGANISMS FOR PRODUCING ISOPROPANOL |
摘要 |
<p>In an embodiment, there is disclosed a recombinant microbial host cell having each of the DNA molecules encoding a polypeptide or group of polypeptides that catalyze the conversion: <?in-line-formulae description="In-line Formulae" end="lead"?>(i) Acetyl-CoA to Acetate and CoA (conversion 1)<?in-line-formulae description="In-line Formulae" end="tail"?> <?in-line-formulae description="In-line Formulae" end="lead"?>(ii) Acetyl-CoA to Acetoacetyl-CoA and CoA (conversion 2)<?in-line-formulae description="In-line Formulae" end="tail"?> <?in-line-formulae description="In-line Formulae" end="lead"?>(iii) Acetoacetyl-CoA and Acetate to Acetoacetate and Acetyl-CoA (conversion 3.1)<?in-line-formulae description="In-line Formulae" end="tail"?> <?in-line-formulae description="In-line Formulae" end="lead"?>(iv) Acetoacetate to Acetone and CO2 (conversion 4)<?in-line-formulae description="In-line Formulae" end="tail"?> <?in-line-formulae description="In-line Formulae" end="lead"?>(v) Acetone and NAD(P)H and H+ to Isopropanol and NAD(P)+ (conversion 5)<?in-line-formulae description="In-line Formulae" end="tail"?> wherein at least one DNA molecule is heterologous to the microbial host cell and wherein the microbial host cell produces isopropanol. In another embodiment, a method is disclosed for the production of isopropanol including providing a recombinant microbial host cell, the host cell of (i) with a fermentable carbon substrate in a fermentation medium under conditions whereby isopropanol is produced, and recovering the isopropanol.</p> |
申请公布号 |
EP2147111(A1) |
申请公布日期 |
2010.01.27 |
申请号 |
EP20080746347 |
申请日期 |
2008.04.18 |
申请人 |
GEVO, INC. |
发明人 |
SUBBIAN, EZHILKANI;MEINHOLD, PETER;BUELTER, THOMAS;HAWKINS, ANDREW C. |
分类号 |
C12P7/66;C12N1/20;C12P7/04 |
主分类号 |
C12P7/66 |
代理机构 |
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代理人 |
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