ACOUSTIC PERFUSION DEVICES

摘要

Acoustic perfusion devices for separating biological cells from other material in a fluid mixture are disclosed. The devices include an inlet port, an outlet port, and a collection port that are connected to an acoustic chamber. An ultrasonic transducer creates an acoustic standing wave in the acoustic chamber that permits a continuous flow of fluid to be recovered through the collection port while keeping the biological cells within the acoustic chamber to be returned to the bioreactor from which the fluid mixture is being drawn.

主权项

1. An acoustic perfusion device, comprising:
an acoustic chamber; an inlet port and an inlet flow path leading from the inlet port to the acoustic chamber; an outlet port and an outlet flow path leading from the acoustic chamber to the outlet port for recirculating a fluid mixture; at least one collection port for collecting harvest fluid from the fluid mixture; and at least one ultrasonic transducer and at least one reflector opposite the at least one ultrasonic transducer, wherein the at least one ultrasonic transducer includes a piezoelectric material driven by a voltage signal to create a multi-dimensional acoustic standing wave across a collection flow path leading from the acoustic chamber to the at least one collection port.