发明名称 |
Method for gene screening with the use of nuclear receptors |
摘要 |
A system in which a ligand is formed by the expression of a polypeptide that converts a ligand precursor into a ligand, and the ligand thus formed binds to a nuclear receptor to thereby induce the expression of a reporter gene located downstream of the target sequence is constructed. Searching a gene library using this system can isolate a gene encoding a polypeptide capable of converting a ligand precursor into a ligand. This system, which takes the advantage of the transcriptional regulatory function of a nuclear receptor, enables screening a ligand that binds to a nuclear receptor and to examine whether or not a test compound is a ligand that binds to the nuclear receptor, and also screening genes that encode polypeptides capable of converting an inactive form of a wide range of transcriptional regulatory factors into an active form. |
申请公布号 |
AU8356498(A) |
申请公布日期 |
1999.02.16 |
申请号 |
AU19980083564 |
申请日期 |
1998.07.22 |
申请人 |
CHUGAI SEIYAKU KABUSHIKI KAISHA |
发明人 |
SHIGEAKI KATO;KENICHI TAKEYAMA;SACHIKO KITANAKA |
分类号 |
C07K14/705;C12N9/02;C12N15/10;C12N15/60;C12N15/63;C12P21/08;C12Q1/68 |
主分类号 |
C07K14/705 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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