Magnetic nanoparticles coupled to annexine, and utilization thereof

摘要

PCT No. PCT/FR96/00964 Sec. 371 Date Jun. 11, 1998 Sec. 102(e) Date Jun. 11, 1998 PCT Filed Jun. 20, 1996 PCT Pub. No. WO97/01760 PCT Pub. Date Jan. 16, 1997The invention relates to a process for producing ferrofluid-forming magnetic particles substituted by DMSA (FFSH) that can be covalently coupled, directly or through a difunctional reactant, to an effector, which process comprises combining DMSA with ferrofluid-forming magnetic particles to form FFSS particles having disulfide bonds, and reducing the disulfide bonds of the FFSS at basic pH to form FFSH. The invention includes a composition comprising such FFSH magnetic particles. The invention also relates to a method in which FFSH magnetic particles are incubated with an effector comprising at least one functional group which forms an S-S, C-S, C-C, or C-N bond with DMSA, so that a covalent effector-FFSH complex is formed. The invention further includes a method for separating and/or distinguishing between molecules or cells having a ligand that forms an affinity complex with an effector-FFSH particle of the invention, and those molecules or cells that do not have such a ligand.