Cell Washing Plunger Using Centrifugal Force

摘要

Devices and methods for washing a suspension of cells are provided. The devices include concentric center, intermediate, and outer compartments. The center compartment and the intermediate compartment are in communication with each other by a first valve and the intermediate and outer compartments are in communication with each other by an opening, aperture, or second valve. The methods include loading a suspension of cells into the center compartment, loading a wash solution into the intermediate compartment, and rotating the device. Centripetal force forces cells through the wash solution and into the outer compartment, where they are isolated and collected.

主权项

1. A device for washing a suspension of cells, the device comprising:
a cylindrical inner wall extending about and along a longitudinal axis from a first end to a second end, the inner wall defining a central compartment; a cylindrical intermediate wall extending about and along the longitudinal axis from the first end to the second end, the cylindrical wall defining an intermediate compartment adjacent to the central compartment; a cylindrical outer wall extending about and along the longitudinal axis from the first end to a third end, the cylindrical outer wall defining an outer compartment adjacent to the intermediate compartment; a first valve positioned through the inner wall at or near the second end such that the first central compartment is in fluid communication with the second intermediate compartment when the valve is open; and an aperture positioned through the intermediate wall at or near the first end such that the second intermediate compartment is in fluid communication with the third outer compartment.