Cell preparation containing mesenchymal stem cells, and method for producing same

摘要

A cell preparation containing mesenchymal stem cells whose immunosuppression ability is maintained is produced by means of a serum-free or low-serum culture. A method for producing a cell preparation containing mesenchymal stem cells, comprising the steps of: (A) proliferating mesenchymal stem cells in a serum-free medium “A” containing an FGF, a PDGF, a TGF-β, an HGF, an EGF, at least one phospholipid, and at least one fatty acid; and (B) screening mesenchymal stem cells whose immunosuppression ability is maintained or improved, from the mesenchymal stem cells thus proliferated in the step (A).

主权项

1. A method for producing a cell preparation containing mesenchymal stem cells, comprising the steps of:
a proliferation step comprising proliferating mesenchymal stem cells in a serum-free medium containing a fibroblast growth factor (FGF), a platelet derived growth factor (PDGF), a transforming growth factor-β (TGF-β), a hepatocyte growth factor (HGF), an epidermal growth factor (EGF), at least one phospholipid, and at least one fatty acid; and a screening step comprising screening mesenchymal stem cells whose immunosuppression ability is maintained or improved, from the mesenchymal stem cells thus proliferated in the proliferation step; and a pre-proliferation step comprising before the proliferation step, culturing the mesenchymal stem cells in a serum-free medium B containing a FGF, a PDGF, an EGF, at least one phospholipid, and at least one fatty acid, the serum-free medium B does not contain a HGF and a TGF-β, a culture time period of the pre-proliferation step and the proliferation step being 48 days to 68 days, and the cell preparation comprises the mesenchymal stem cells whose immunosuppression ability is maintained or improved; wherein the mesenchymal stem cells are subcultured at least once in the pre-proliferation step.