TEST METHOD FOR EVALUATING THE RISK OF ANTI-THYROID DRUG-INDUCED AGRANULOCYTOSIS, AND EVALUATION KIT

摘要

The present invention provides a test method and an evaluation kit for determining the risk of antithyroid drug-induced agranulocytosis. More particularly, it provides a test method for determining the risk of antithyroid drug-induced agranulocytosis, including testing susceptibility polymorphism to antithyroid drug-induced agranulocytosis, and determining the risk of antithyroid drug-induced agranulocytosis, and an evaluation kit for the risk of antithyroid drug-induced agranulocytosis, containing a polynucleotide capable of detecting susceptibility polymorphism to antithyroid drug-induced agranulocytosis.

主权项

1. A test method for determining the risk of antithyroid drug-induced agranulocytosis, comprising
(1) a step of using a sample derived from a test subject and testing polymorphism present in the HLA region, which is at least one selected from the group consisting of
A) polymorphism at the 501st nucleotide in the nucleotide sequence shown in SEQ ID NO: 1 (G>T*),B) polymorphism at the 201st nucleotide in the nucleotide sequence shown in SEQ ID NO: 2 (C>T*),C) polymorphism at the 501st nucleotide in the nucleotide sequence shown in SEQ ID NO: 3 (C>T*),D) polymorphism at the 501st nucleotide in the nucleotide sequence shown in SEQ ID NO: 4 (T*>G),E) polymorphism at the 501st nucleotide in the nucleotide sequence shown in SEQ ID NO: 5 (C>T*), wherein parentheses show reference allele>variant allele, * is risk allele, G, A, T and C are guanine, adenine, thymine and cytosine, respectively, and
F) polymorphism in linkage disequilibrium with the polymorphism of any of the above-mentioned A)-E), the linkage disequilibrium showing a linkage disequilibrium coefficient D′ of not less than 0.8, and (2) a step of determining the risk of antithyroid drug-induced agranulocytosis based on the test results of (1).