| 摘要 |
<p>The object is to develop: a novel vector, preferably a novel vector which can be carried in a cell of a bacterium belonging to the genus Ralstonia, Cupriavidus or Wautersia stably in the absence of selection pressure for an antibiotic and has no conjugal transfer property; a strain capable of stably producing a polyhydroxyalkanoate using the vector; and a method for production of a polyhydroxyalkanoate using the strain. Disclosed is a novel recombinant vector having a DNA replication initiation segment which can function in a cell of a bacterium belonging to the genus Ralstonia, Cupriavidus or Wautersia, particularly a recombinant vector having both a DNA replication initiation segment which can function in a cell of a bacterium belonging to the genus Ralstonia, Cupriavidus or Wautersia and a segment which can stabilize the recombinant vector (a par segment). By using a transformant produced using the vector, the vector can be carried in a bacterium stably and a polyhydroxyalkanoate can be produced with good efficiency.</p> |
