Molecular Conjugate

摘要

A method is disclosed for making a conjugate of two molecules using a hydrazide thiol linker. In a particular working embodiment, an Fc-specific antibody-enzyme conjugate is made using the method and demonstrated to provide exceptional staining sensitivity and specificity in immunohistochemical and in situ hybridization assays.

主权项

1. A conjugate comprising an antibody covalently bonded to a detectable label through a hydrazide thiol linker, wherein the hydrazide thiol linker is one or more of MBH, MBCH, MAMBH, THMBH, BTAL, BTHL, TAGD, THGD, a PEG-based hydrazide-thiol linker, a multifunctional hydrazide thiol linker, a PEG-based multifunctional hydrazide thiol linker, and a polyacrylamide hydrazide thiol linker, wherein a hydrazide group of the linker is covalently bonded to an Fc region of the antibody; and wherein the detectable label is an enzyme having at least one thiol reactive group, wherein a thiol group of the hydrazide thiol linker is coupled to the at least one thiol reactive group of the enzyme.