METHOD AND SYSTEM FOR REAL TIME QUANTIFICATION AND MONITORING OF NUCLEIC ACID AMPLIFICATION USING ELECTROCONDUCTIVE OR ELECTROCHEMICALLY ACTIVE LABELS

摘要

A method and device for real time electrochemically or electrically monitoring and detecting nucleic acid amplification products, i.e. after each polymerase chain reaction cycle, utilizes electrochemically active or electrically conductive reporter materials. An electric voltage is applied and electric signals are measured during a PCR amplification process to the electrodes that is suitable for quantifying the amplified products of a sample's nucleic acid(s) produced. This technique is suitable for point-of-use applications, e.g. detecting bioanalytes in remote locations.