PROTEIN GLYCOSYLATION MODIFICATION IN <i>PICHIA PASTORIS</i>

摘要

The present invention provides genetically engineered strains of Pichia capable of producing proteins with reduced glycosylation. In particular, the genetically engineered strains of the present invention are capable of expressing either or both of an alpha -1,2-mannosidase and glucosidase II. The genetically engineered strains of the present invention can be further modified such that the OCH1 gene is disrupted. Methods of producing glycoprotiens with reduced glycosylation using such genetically engineered stains of Pichia are also provided.