Regeneration Of A Chromatography Matrix

摘要

The present invention relates to a process of regenerating a separation matrix, such as a chromatography matrix, comprising adsorption of at least one target molecule by contacting a mobile phase comprising at target molecule(s) with a matrix; removal of unbound material by washing the matrix; elution of target molecule(s) by contacting the matrix with an eluent; reducing regeneration by contacting said matrix with a reducing agent; alkaline regeneration by contacting the matrix with an alkaline solution; and equilibration of the matrix.