| 摘要 |
PROBLEM TO BE SOLVED: To obtain cultured skin that has a specific water permeability coefficient and is useful in metabolism test of medicines and cosmetics by culturing epithelial keratinocytes in a culture medium containing ascorbic acid and blood serum and specific substances as vitamins and hormones. SOLUTION: A culture medium containing ascorbic acid, serum, in addition, biotin, choline, pantothenic acid, tocopherol and carnitine as vitamins, and dexamethasone, progesterone and isoproterenol as hormones is used and collagen gel is used as a support to sew epithelial keratinocytes and the objective cultured skin is obtained thereby including the epithelial keratinocyte culture mixture with a water permeability of <=10×10-13 cm/h at 20-25 deg.C. This cultured skin is useful in percutaneous absorption test and the like that can reproduce the permeability of human skin by improving the skin permeability and is suitable for the metabolism tests of medicines and cosmetics, particularly skin permeation of medicines. |
