GENERATION OF ANTERIOR FOREGUT ENDODERM FROM PLURIPOTENT CELLS

摘要

The invention is directed to in vitro methods of inducing differentiation of anterior foregut endoderm and the enriched populations of anterior foregut endoderm produced by such methods. Such enriched populations are useful for studies of the molecular events that occur during differentiation and for generating cells for cell replacement therapy.

主权项

1. A method of preparing an enriched population of anterior foregut endoderm cells from pluripotent cells, the method comprising:
(i) culturing the pluripotent cells under conditions that induce the pluripotent cells to form definitive endoderm cells; and (ii) culturing the definitive endoderm cells under conditions that induce the definitive endoderm cells to form anterior foregut endoderm cells, thereby preparing the enriched population of anterior foregut endoderm cells.