| 摘要 |
A process for generating a synthetic engineered recombinant protein has been performed by providing an original protein design and the necessary probabilistic priors for random in silico assembly. Once the in silico synthetic protein is assayed using mathematic modeling, the proteins are then reverse translated into DNA. The DNA fragment, modifying the original DNA to obtain a first DNA fragment, wherein the modifying step comprising adding an antigen optimization sequence to the original DNA fragment, purifying the first DNA fragment, performing a DNA self-assembly or a ligation, adding an amplification linker to obtain a second DNA fragment, purifying the second DNA fragment, transferring the second DNA fragment into an expression vector, expressing the second DNA fragment into a synthetic engineered recombinant protein, as well as purifying the synthetic engineered recombinant protein. |
