发明名称 |
RNA-GUIDED SYSTEMS FOR PROBING AND MAPPING OF NUCLEIC ACIDS |
摘要 |
Methods of detecting, probing, mapping and directed sequencing of target nucleic acids are provided using a guide RNA and a Cas9 protein. Methods for detecting the binding of the guide RNA/Cas9 complex to a target nucleic acid where the guide RNA includes a 3' tail sequence that can hybridize to a probe are provided. Methods for detecting the binding of the guide RNA/Cas9 complex to a target nucleic acid where the complex is physically detected are provided. |
申请公布号 |
WO2016028843(A3) |
申请公布日期 |
2016.07.14 |
申请号 |
WO2015US45805 |
申请日期 |
2015.08.19 |
申请人 |
PRESIDENT AND FELLOWS OF HARVARD COLLEGE |
发明人 |
CHURCH, GEORGE M.;VIGNEAULT, FREDERIC;MIR, KALIM U. |
分类号 |
C12P19/34;C07H21/02;C12Q1/68 |
主分类号 |
C12P19/34 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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