FLOW CELL FOR NUCLEIC ACID ANALYSIS AND NUCLEIC ACID ANALYZER

摘要

Provided is a flow cell for nucleic acid analysis used for a sequence reaction with photocleavable nucleotides where an efficiency of the photocleaving reaction can be enhanced and noise upon florescence detection can be mitigated, thereby improving the accuracy of sequencing, shortening a runtime, and extending a read length. The flow cell for nucleic acid analysis includes a first substrate 101 provided with an optical filter 102 reflecting first light for changing a chemical structure of a substance in a flow passage, a hollow sheet 103 having a hollow portion for forming the flow passage, and a second substrate 105 transmitting the first light, in which the first substrate, the hollow sheet, and the second substrate are attached to each other.

主权项

1. A flow cell for nucleic acid analysis, comprising: a first substrate provided with an optical filter for reflecting first light for carrying out a photo-cleaving reaction with a nucleotide having an intramolecular florescent molecule via a photocleavable linker; a hollow sheet having a hollow portion for forming a flow passage; and a second substrate transmitting the first light, wherein the first substrate, the hollow sheet, and the second substrate are attached to each other,
wherein a plurality of bead carriers, a surface of which carries a plurality of molecules of the nucleotide having the intramolecular florescent molecule via the photocleavable linker, is fixed inside the flow passage, and the optical filter transmits second light for exciting the florescent molecule and third light irradiated from the florescent molecule.