| 发明名称 | Optimized endonucleases and uses thereof | ||
| 摘要 | Provided are optimized endonucleases, as well as methods of targeted integration, targeted deletion or targeted mutation of [polynucleotides using optimized endonucleases. | ||
| 申请公布号 | US9404099(B2) | 申请公布日期 | 2016.08.02 |
| 申请号 | US201013512219 | 申请日期 | 2010.11.25 |
| 申请人 | BASF Plant Science Company GmbH | 发明人 | Hlubek Andrea;Biesgen Christian |
| 分类号 | C12N9/22;C12N5/14;C12N15/82 | 主分类号 | C12N9/22 |
| 代理机构 | Drinker Biddle & Reath LLP | 代理人 | Drinker Biddle & Reath LLP |
| 主权项 | 1. An optimized endonuclease comprising an amino acid sequence having at least 92% sequence identity to the amino acid sequence of SEQ ID NO: 1 or 2, wherein said optimized endonuclease does not comprise the amino acid sequence TISSETFLK (SEQ NO: 31) at its C-terminus and has a higher capability to induce homologous recombination in comparison to an endonuclease having an identical amino acid sequence but comprising the amino acid sequence TISSETFLK (SEQ ID NO: 31) at its C-terminus. | ||
| 地址 | Ludwigshafen DE | ||