T cell receptor display

摘要

A proteinaceous particle, for example a bacteriophage, ribosome or cell, displaying on its surface a T-cell receptor (TCR). The displayed TCR is preferably a heterodimer having a non-native disulfide bond between constant domain residues. Such display particles may be used for the creation of diverse TCR libraries for the identification of high affinity TCRs. Several high affinities are disclosed.

主权项

1. A phage particle displaying on its surface a dimeric T-cell receptor (dTCR) polypeptide pair, wherein the dTCR polypeptide pair comprises
a first polypeptide wherein a TCR α or δ chain variable domain sequence is fused to N terminus of a TCR αchain constant domain extracellular sequence, and a second polypeptide wherein a TCR β or γ chain variable domain sequence is fused to N terminus of a TCR β chain constant domain extracellular sequence,wherein the first and second polypeptides are linked by a disulfide bond which corresponds to a native inter-chain disulfide bond present in native dimeric αβ TCRs.