GM1 ganglioside to annexin V microparticle polypeptide ratio for biological monitoring

摘要

We describe a method of monitoring the state of a cell, tissue, organ or organism. The method comprises establishing, for a sample of microparticles from the cell, tissue, organ or organism, a ratio. The ratio is of a selected polypeptide in microparticles which comprise GM1 gangliosides, preferably which bind to Cholera Toxin B (CTB) (“GM1 ganglioside microparticle polypeptide”) to the selected polypeptide in microparticles which comprise exposed phosphotidylserine, preferably which bind to Annexin V (“Annexin V microparticle polypeptide”). The GM1 ganglioside microparticle polypeptide to Annexin V microparticle polypeptide ratio so established may be indicative of the state of the cell, tissue, organ or organism.

主权项

1. A method of monitoring the state of a cell, tissue, organ or organism, and treating a cardiovascular disease state, the method comprising establishing, for a sample of microparticles from the cell, tissue, organ or organism, a ratio of:
(i) (a) a selected polypeptide in microparticles which comprise GM1 gangliosides to (b) the selected polypeptide in microparticles which comprise exposed phosphotidylserine wherein the (a) to (b) ratio is indicative of the state of the cell, tissue, organ or organism (ii) wherein if the ratio of (a) to (b) is higher in the sample of microparticles relative to a control sample, administering a cardiovascular disease treatment to the cell, tissue, organ or organism.