发明名称 |
GENE-DISRUPTED STRAIN, RECOMBINANT PLASMID, TRANSFORMANT AND METHOD OF PRODUCING 3-CARBOXYMUCONOLACTONE |
摘要 |
Industrial-scale fermentative production of 3-carboxy-cis,cis-muconic acid from terephthalic acid.
Also, a protocatechuate 4,5-ring-cleaving enzyme gene-disrupted strain in which the gene coding for (a) the amino acid sequence set forth in SEQ ID NO: 1 or 3, or (b) the amino acid sequence set forth in SEQ ID NO: 1 or 3 which has a deletion, substitution, addition and/or insertion of one or more amino acids and exhibits protocatechuate 4,5-ring cleavage activity, present in the chromosomal DNA of microbial cells, has been disrupted; recombinant plasmids comprising the Tph gene and protocatechuate 3,4-dioxygenase gene; transformants obtained by introducing the recombinant plasmids into the disrupted strain; and a process for production of 3-carboxy-cis,cis-muconic acid and/or 3-carboxymuconolactone characterized by culturing the transformants in the presence of terephthalic acid. |
申请公布号 |
EP2147973(A1) |
申请公布日期 |
2010.01.27 |
申请号 |
EP20080752842 |
申请日期 |
2008.05.09 |
申请人 |
KABUSHIKI KAISHA TOYOTA JIDOSHOKKI;NATIONAL UNIVERSITY CORPORATION TOKYO UNIVERSITY OF AGRICULTURE AND TECHNOLOGY;NAGAOKA UNIVERSITY OF TECHNOLOGY;FORESTRY AND FOREST PRODUCTS RESEARCH INSTITUTE |
发明人 |
SHIMO, TOSHIHISA;MASE, KOHEI;KATAYAMA, YOSHIHIRO;MASAI, EIJI;FUKUDA, MASAO;SHIGEHARA, KIYOTAKA;OHARA, SEIJI;NAKAMURA, MASAYA;OTSUKA, YUICHIRO |
分类号 |
C12N15/00;C12N1/15;C12N1/19;C12N1/21;C12N5/10;C12N15/09;C12P7/44;C12P17/04 |
主分类号 |
C12N15/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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