| 摘要 |
<p>It is intended to provide a method of extracting a nucleic acid, in particular, free DNA in the blood from a blood component in high yield and easily, and a reagent and a kit to be used for the method, which have excellent storage stability and with which the recovery rate of nucleic acid is stably high. The method of extracting a nucleic acid from a blood component is characterized by comprising the steps of (1) reacting a blood component with a protease in the presence of a surfactant and a salt; (2) bringing the resulting reaction solution into contact with a chaotropic agent and a coprecipitating agent; and (3) performing a treatment for precipitating a nucleic acid. The kit for extracting a nucleic acid from a blood component comprises in combination, (1) a reagent containing a protease; (2) a reagent containing a surfactant and a salt; and (3) a reagent containing a chaotropic agent and a coprecipitating agent. According to the invention, it becomes possible to extract a nucleic acid, in particular free DNA in the blood from a blood component (such as serum or plasma) in high yield and easily, and it becomes possible to improve the storage stability of a reagent to be used for the same.</p> |
