发明名称 |
METHODS FOR CONVERSION OF THE SUBSTRATE SPECIFICITY OF DESATURASES |
摘要 |
The present invention relates to methods for the conversion of the substrate specificity of desaturases. Specifically, the present invention pertains to a method for the conversion of the substrate specificity of a Δ5 and/or Δ6 desaturase to the substrate specificity of a Δ4 desaturase, the method comprising: identifying regions and/or amino acid residues which control the substrate specificity of (i) the Δ5 and/or Δ6 desaturase and (ii) the Δ4 desaturase; and replacing in the amino acid sequence of the mentioned Δ5 and/or Δ6 desaturase, the regions and/or amino acid residues which control the substrate specificity of the Δ5 and/or Δ6 desaturase, by the corresponding regions and/or amino acid residues which control the substrate specificity of the Δ4 desaturase, thereby converting the substrate specificity of the Δ5 and/or Δ6 desaturase to the substrate specificity of the Δ4 desaturase. The present invention further concerns a method for the conversion of the substrate specificity of a Δ4 desaturase to the substrate specificity of a Δ5 and/or Δ6 desaturase, the method comprising: identifying regions and/or amino acid residues which control the substrate specificity of (i) the Δ4 desaturase and (ii) the Δ5 and/or Δ6 desaturase; and replacing in the amino acid sequence of the indicated Δ4 desaturase, the regions and/or amino acid residues which control the substrate specificity of the Δ4 desaturase, by the corresponding regions and/or amino acid residues which control the substrate specificity of the Δ5 and/or Δ6 desaturase, thereby converting the substrate specificity of the Δ4 desaturase to the substrate specificity of the Δ5 and/or Δ6 desaturase. In addition, the invention encompasses desaturases with converted substrate specificity. |
申请公布号 |
US2016319249(A1) |
申请公布日期 |
2016.11.03 |
申请号 |
US201415105080 |
申请日期 |
2014.12.17 |
申请人 |
BASF PLANT SCIENCE COMPANY GMBH |
发明人 |
Senger Toralf;Vrinten Patricia;Lim Ze Long |
分类号 |
C12N9/02;C12P7/64 |
主分类号 |
C12N9/02 |
代理机构 |
|
代理人 |
|
主权项 |
1. A method for the conversion of the substrate specificity of a Δ5 and/or Δ6 desaturase to the substrate specificity of a Δ4 desaturase, the method comprising:
a) identifying regions and/or amino acid residues which control the substrate specificity of
(i) the Δ5 and/or Δ6 desaturase and (ii) the Δ4 desaturase; and b) replacing in the amino acid sequence of the Δ5 and/or Δ6 desaturase referred to in step a), the regions and/or amino acid residues which control the substrate specificity of the Δ5 and/or Δ6 desaturase, by the corresponding regions and/or amino acid residues which control the substrate specificity of the Δ4 desaturase, thereby converting the substrate specificity of the Δ5 and/or Δ6 desaturase to the substrate specificity of the Δ4 desaturase. |
地址 |
Ludwigshfen DE |