| 发明名称 |
Polymerase Variants and Uses Thereof |
| 摘要 |
Described herein is a variant pol6 polymerase having at least one mutation selected from H223, N224, Y225, H227, 1295, Y342, T343, I357, S360, L361, I363, S365, S366, Y367, P368, D417, E475, Y476, F478, K518, H527, T529, M531, N535, G539, P542, N545, Q546, A547, L549, I550, N552, G553, F558, A596, G603, A610, V615, Y622, C623, D624, I628, Y629, R632, N635, M641, A643, I644, T647, I648, T651, I652, K655, W656, D657, V658, H660, F662, L690 and combinations thereof. |
| 申请公布号 |
US2016333327(A1) |
申请公布日期 |
2016.11.17 |
| 申请号 |
US201615151364 |
申请日期 |
2016.05.10 |
| 申请人 |
Genia Technologies, Inc. |
发明人 |
AYER Aruna;ARNOLD Cleoma;SCHWAB Charles Wayan;THAI Eileen;LEDERMAN Ilya;MCGAW Colin Alexander;SHULTZ Tyler O'Brien |
| 分类号 |
C12N9/12 |
主分类号 |
C12N9/12 |
| 代理机构 |
|
代理人 |
|
| 主权项 |
1. A modified DNA polymerase having a DNA polymerase activity comprising an amino acid sequence having at least 70% sequence identity to the amino acid sequence as set forth in SEQ ID NO: 1 or 2, which amino acid sequence includes one or more amino acid substitutions selected from the group consisting of H223, N224, Y225, H227, I295, Y342, T343, I357, S360, L361, I363, S365, S366, Y367, P368, D417, E475, Y476, F478, K518, H527, T529, M531, N535, G539, P542, N545, Q546, A547, L549, I550, N552, G553, F558, A596, G603, A610, V615, Y622, C623, D624, I628, Y629, R632, N635, M641, A643, I644, T647, I648, T651, I652, K655, W656, D657, V658, H660, F662, L690, and combinations thereof. |
| 地址 |
Santa Clara CA US |
