ARRANGEMENT FOR LIGHT SHEET MICROSCOPY

摘要

The invention is directed to an arrangement for light sheet microscopy. An arrangement of this type comprises illumination optics with an illumination objective (5) for illuminating a sample (3) with a light sheet via an illumination beam path, which sample (3) is located on a sample carrier in a medium (2), wherein the optical axis (6) of the illumination objective (5) and the light sheet lie in a plane which forms an illumination angle (β) not equal to zero with the normal lines of a plane reference surface (4) with respect to which the sample carrier is aligned. The arrangement further comprises detection optics with a detection objective (7) in a detection beam path, the optical axis (8) of the detection objective (7) forming a detection angle (δ) not equal to zero with the normal lines of the reference surface (4). Finally, the arrangement also comprises a separating layer system with at least one layer made of a predetermined material with predetermined thickness which separates the medium (2) from the illumination objective (5) and the detection objective (7), wherein the separating layer system is aligned with a base surface (9) parallel to the reference surface (4) and contacts the medium (2) by this base surface (9) at least in the region which is accessible to the illumination objective (5) and the detection objective (7) for illumination and detection. The arrangement further comprises a corrective lens system with at least one detection corrective lens for reducing those aberrations occurring as a result of light to be detected passing obliquely through interfaces of the separating layer system. In an arrangement of this type, the at least one detection corrective lens (9) is configured as free-form lens and is arranged between the detection objective (7) and the separating layer system. Alternatively, it also forms the front lens of the detection objective (7).

主权项

1: An arrangement for light sheet microscopy comprising;
illumination optics comprising:
an illumination objective configured to illuminate a sample with a light sheet via an illumination beam path, when the sample is located in a medium on a sample carrier that is aligned with respect to a plane reference surface;wherein an optical axis of the illumination objective and the light sheet lies in a plane which forms an illumination angle not equal to zero with normal lines of the plane reference surface; detection optics comprising:
a detection objective in a detection beam path;wherein and optical axis of the detection objective forms a detection angle not equal to zero with the normal lines of the reference surface; a separating layer system comprising:
at least one layer made of a predetermined material with a predetermined thickness which separates the medium from the illumination objective and the detection objective;wherein the separating layer system configured to be aligned with and contact the medium by a base surface parallel to the reference surface at least in a region that is accessible by the illumination objective and the detection objective for illuminating and detecting; and a detection corrective lens system comprising:
at least one detection corrective lens configured to reduce aberrations that occur as a result of light to be detected passing obliquely through interfaces of the separating layer system;wherein the at least one detection corrective lens is configured as a free-form lens, and is arranged between the detection objective and the separating layer system or forms the a front lens of the detection objective.