POLYCLONAL MIXTURES OF ANTIBODIES, AND METHODS OF MAKING AND USING THEM

摘要

A method of broadening epitopic coverage of an antigen of interest, wherein a first sample of the antigen of interest is contacted with a first plurality of host cells collectively expressing a first library of antibodies. Host cells expressing antibodies that bind to the antigen are then collected from among the first plurality of host cells, and a composition is prepared comprising a polyclonal mixture of antibodies expressed by these host cells. A second sample of the antigen of interest is then contacted with an aliquot of the prepared composition and a second plurality of host cells collectively expressing a second library of antibodies. Host cells expressing antibodies that bind to the second sample of the antigen are then collected from among the second plurality of host cells.

主权项

1. A method of broadening epitopic coverage of an antigen of interest, the method comprising:
a) contacting a first sample of the antigen of interest with a first plurality of host cells collectively expressing a first library of antibodies; b) collecting host cells expressing antibodies that bind to the antigen from among the first plurality of host cells employed in step a); c) preparing a composition comprising a polyclonal mixture of antibodies expressed by host cells collected in step b); d) contacting a second sample of the antigen of interest with an aliquot of the composition prepared in step c) and a second plurality of host cells collectively expressing a second library of antibodies; and e) after performing step d), collecting host cells from among the second plurality of host cells expressing antibodies that bind to the second sample of the antigen from among the second plurality of host cells; wherein an increase in the number of different epitopes collectively recognized by the antibodies expressed by the host cells collected in step c) relative to the number of different epitopes collectively recognized by the antibodies contained in the composition prepared in step b) indicates that epitopic coverage of the antigen has been broadened.