CELLULOSE- AND HEMICELLULOSE-DEGRADATION ENZYME -ENCODING NUCLEOTIDE SEQUENCES WITH REFINED TRANSLATIONAL KINETICS AND METHODS OF MAKING SAME
摘要
Provided are polynucleotide sequences and synthetic genes encoding cellulose- and hemicellulose-degradation enzymes for expression in a host organism with improved and/or refined translational kinetics, and methods of making same. The resultant cellulose- and hemicellulose-degradation enzyme-encoding nucleotide is predicted to be translated rapidly along its entire length. Expression of the resultant cellulose- and hemicellulose-degradation enzyme-encoding nucleotide is predicted to result in improved protein expression levels in cases where inappropriate or excessive translation pauses reduce protein expression. In addition, expression of the resultant cellulose- and hemicellulose-degradation enzyme-encoding nucleotide is predicted to result in improved levels of active and/or natively folded and functional polypeptide expression in cases where inappropriate or excessive translational pauses causes expression of inactive, insoluble, aggregated or somehow dysfunctional or minimally active cellulose- and hemicellulose-degradation enzyme.
申请公布号
WO2009005564(A3)
申请公布日期
2009.03.05
申请号
WO2008US06379
申请日期
2008.05.14
申请人
THE REGENTS OF THE UNIVERSITY OF CALIFORNIA;SALMON, KIRSTY, A.;ROTH, DAVID, A.;HATFIELD, WESLEY, G.;DOU, YIMENG