| 主权项 |
1. A method comprising the follow steps:
(i) obtaining a biological sample from a patient who does not have severe periodontal disease; (ii) isolating a nucleic acid sample from said biological sample; (iii) contacting said nucleic acid sample with oligonucleotide primers that hybridize 5′ and 3′ to each of the following single nucleotide polymorphisms (SNPs) in the IL1B gene: rs16944 C/T; rs1143623 G/C; and rs4848306 C/T; (iv) amplifying nucleic acid in said nucleic acid sample with said oligonucleotide primers to produce one or more amplified fragments, wherein each of said SNPs is included in at least one of said one or more amplified fragments; (v) detecting the number of copies of allele 1 and allele 2 of each of said SNPs in said one or more amplified fragments, wherein as a result of said detecting, the presence of one of the following composite genotypes is detected in said patient:
a) one copy rs16944 allele 1 (C), one copy rs1143623 allele 1 (G), and two copies of rs4848306 allele 1 (C);b) two copies of rs16944 allele 2 (T), one copy of rs1143623 allele 1 (G), and two copies of rs4848306 allele 1 (C);c) two copies of rs16944 allele 1 (C), two copies of rs1143623 allele 1 (G), and two copies of rs4848306 allele 1 (C);d) one copy of rs16944 allele 1 (C), two copies of rs1143623 allele 1 (G), and two copies of rs4848306 allele 1 (C);e) two copies of rs16944 allele 2 (T), two copies of rs1143623 allele 1 (G), and two copies of rs4848306 allele 1 (C); and (vi) treating the patient of step (v), in whom one of the composite genotypes a)-e) has been detected, with a treatment regimen to slow down the progression of periodontal disease. |
