PROCESS FOR THE ENZYMATIC PRODUCTION OF 1-BUTENE FROM 2-PENTENOYL-COA

摘要

Described is a method for the production of 2-pentenoic acid comprising the enzymatic conversion of 2-pentenoyl-CoA into 2-pentenoic acid comprising two enzymatic steps comprising (i) first enzymatically converting 2-pentenoyl-CoA into 2-pentenoyl phosphate; and (ii) then enzymatically converting the thus obtained 2-pentenoyl phosphate into said 2-pentenoic acid; or a single enzymatic reaction in which 2-pentenoyl-CoA is directly converted into 2-pentenoic acid by making use of a CoA transferase (EC 2.8.3.-), preferably a butyryl-CoA:acetate-CoA transferase (EC 2.8.3.8).