Isothermal nucleic acid amplification reactor with integrated solid state membrane

摘要

Provided are devices adapted to isolate, amplify, and detect nucleic acids that may be present in a biological sample. The devices can, in some embodiments, isolate, amplify, and detect nucleic acid in a single chamber. In other embodiments, the devices are adapted to isolate and amplify nucleic acids in a reaction chamber, after which the nucleic acids may be communicated to a pervious medium—such as a lateral flow strip—where the user may label and detect the nucleic acids.

主权项

1. An analysis device, comprising:
a chamber having a first and a second outlet; a capture material that preferentially binds nucleic acids, the capture material being positioned such that the capture material is capable of fluid communication with the interior of the chamber; a first pervious body adapted such that the first pervious body and the chamber are capable of relative motion such that the first pervious body is placed into fluid communication with the chamber; a pervious transport material disposed at an outlet of the chamber; and a second pervious body, the second pervious body being positionable such that the second pervious body contacts the transport material.