摘要 |
PROBLEM TO BE SOLVED: To provide a method for producing recombinant collagen-like polypeptide having high productivity, and to provide the polypeptide and homogenous AgX emulsion materials for photographic applications that use the same. SOLUTION: The present invention relates to a tabular silver halide emulsion, wherein the tabular grains account for 75% or higher of the total grain projected area, the emulsion having silver halide grains nucleated in the presence of nucleation peptizer and thereafter grown in the presence of growth peptizer, wherein at least one of the peptizers is substantially pure collagen-like material prepared by genetic engineering, the peptizer having an amino acid sequence comprising more than 4 different amino acids. A method of preparing the AgX emulsion is also provided. A method of producing recombinant collagen-like polypeptide, having expression of a collage-like polypeptide-encoding nucleic acid sequence by a microorganism to a degree exceeding 0.95 grams/liter, is also provided, with the recombinant collagen being free of a helix structure, and preferably, the expression occurs in microorganisms other than E. coli or Saccaromyces cerevisiae. COPYRIGHT: (C)2009,JPO&INPIT |