METHODS, COMPOSITIONS, AND KITS FOR NUCLEIC ACID ANALYSIS

摘要

Aspects of the invention relate to methods and kits for assessing cancer. Some aspects of the invention relate to methods and kits for preparing a sample library for sequencing. Some aspects of the invention relate to methods and kits for allele detection. Some aspects of the invention relate to high efficiency ligation methods and kits. Some aspects of the invention relate to sensitive detection of amplicons.

主权项

1. A method comprising ligating single-stranded donor nucleic acid molecules to single-stranded acceptor nucleic acid molecules, wherein said single-stranded donor nucleic acid molecules or said single-stranded acceptor nucleic acid molecules comprise a size of 50-2000 nucleotides, wherein an efficiency of said ligating said single-stranded stranded donor nucleic acid molecules is over 10%.