METHOD FOR CULTURING PLURIPOTENT STEM CELLS, AND FACILITY THEREFOR

摘要

In order to culture the pluripotent stem cell which can be variously differentiated, while preventing the cross contamination between the different cells and securing the safety of the products; extending a main transport path 31 outwardly from a stem cell conditioning area 20, the stem cell conditioning area 20 including a treatment room 21 for inducing pluripotent stem cell from a somatic cell or an egg cell, or a treatment room 21 for receiving and conditioning pluripotent stem cell induced in other facilities; branching at least one branched transport path 32 from the main transport path 31; and arranging along each branched transport path 32, a cell culture area 40 including culture rooms 41 to 44 for culturing the stem cell and an analysis room 45 for analyzing the cultured cell, respectively. Preferably, the stem cell conditioning area 20, the cell culture area 40 and a transport area 30 including the main transport path 31 and the branched transport path 32 are provided with respective operator gates 22, (47+48), 33 individually, so as to prohibit coming and going of operator among the areas 20, 40, 30.

主权项

1. A method for culturing pluripotent stem cell comprising the steps of:
extending a main transport path outwardly from a stem cell conditioning area, the stem cell conditioning area including a treatment room for inducing pluripotent stem cell from a somatic cell or an egg cell, or a treatment room for receiving and conditioning pluripotent stem cell induced in other facilities; branching at least one branched transport path from the main transport path; and arranging, along each branched transport path, a cell culture area including a culture room for culturing the stem cell and an analysis room for analyzing the cultured cell, respectively.