METHODS AND KITS FOR LABELING, DETECTION AND ISOLATION OF FOXP3+ REGULATORY T CELLS, ISOLATED POPULATION OF FOXP3+ REGULATORY T CELLS THUS OBTAINED AND USES THEREOF

摘要

The invention relates to a method for labeling, detecting and/or isolating Foxp3+ Treg cells from a biological sample containing peripheral blood mononuclear cells (PBMC) or lymphocytes comprising the following steps of: (i) coupling the surface of PBMC or lymphocytes to a capture moiety which binds to the cell through a cell surface molecule and to interleukin-34 (IL34), (ii) culturing the lymphocytes under conditions wherein IL34 is secreted, released and specifically captured by the capture moiety, (iii) labeling the IL34 expressing lymphocytes with a label moiety, and (iv) optionally detecting and/or isolating said IL34 expressing lymphocytes which are Foxp3+ Treg cells. The invention also relates to an isolated population of Foxp3+ Treg cells obtainable by a method of the invention and uses thereof.