| 摘要 |
Described herein are methods for producing structures useful, for example, for tissue engineering. In their most general form, the methods described herein involve an enzyme activity immobilized on a surface, which is placed in contact with a solution comprising polypeptide monomers. The activity of the enzyme modifies the polypeptide monomers to promote polymerization of the monomers to form polymer structures associated with the surface. Thus, the methods described herein provide a guided self-assembly of polypeptide structures. In preferred embodiments, the self assembly is guided to form regular lattices or other specific structures by specifying the arrangement of the surfaces to which the enzyme, optionally with one or more co-factors, is attached.
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