| 摘要 |
Efficient simultaneous laser irradiation fluorescence detection is performed for a plurality of channels of a microchip and simple and highly sensitive parallel analysis of a plurality of samples is enabled. In a microchip including a material m1 having a refractive index n1, a plurality of channels filled with materials m2 having a refractive index n2 and a plurality of channels filled with materials m3 having a refractive index n3 are alternately arranged in parallel on the same plane. Here, m1, m2, and m3 are selected such that a relation of n2<n1<n3 is satisfied. m2 is a transparent liquid suitable for targeted analysis. If a laser beam is irradiated vertically to the individual channels and along an array plane, in a state in which the laser beam is narrowed, a refraction function by the channel and a refraction function by the channel for the laser beam are offset. For this reason, the laser beam propagates linearly on the array plane and the individual channels and can be simultaneously irradiated with the laser beam efficiently. Fluorescence emitted from the individual channels and is detected independently and simultaneously using a collimating lens, a filter and diffraction grating, an imaging lens, a two-dimensional sensor, and a data analysis system, from a direction vertical to the array plane. |
