发明名称 |
Competitive enzyme linked immunosorbent assay (C-ELISA) for the detection of a flavivirus specific antibody |
摘要 |
<p>A competitive enzyme-linked immunosorbent assay (C-ELISA), using flavivirus member specific immunological agents was developed to detect antibody specific to members of the flaviviruses indicative of exposure to flavivirus. The test is based on a competition for epitope binding on the envelope protein of the flavivirus antigen captured using anti-flavivirus IgA in the presence of flavivirus positive serum. This test has comparable sensitivity specificity and speed to the virus neutralization assay (VNT). C-ELISA is a versatile technique, which could have various applications. Slight modifications of this protocol could lead to a C-ELISA-based detection method of secondary infection or one that could be used for serotype specific sero-epidemiological studies and/or vaccine evaluation. The protocol developed for C-ELISA was demonstrated using dengue lysate antigen and dengue specific monoclonal antibody. This can be used against other flaviviruses and the results for Japanese encephalitis illustrates this.</p> |
申请公布号 |
AU2007225457(A1) |
申请公布日期 |
2007.09.20 |
申请号 |
AU20070225457 |
申请日期 |
2007.03.16 |
申请人 |
NATIONAL ENVIRONMENT AGENCY |
发明人 |
BIJON KUMARSIL;CHENNY SHI CHENG LI |
分类号 |
G01N33/569;C07K1/14;C07K1/22;C07K2/00;C12N7/02 |
主分类号 |
G01N33/569 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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