PREPARATIVE CHROMATOGRAPHY COLUMN

摘要

A chromatography column design and a packing device, as well as a method for obtaining a column with a very compact and uniform bed are provided. The bed remains compact and uniform during operation of the column and during sanitization, storage and transportation of the column. The amount of hardware, particularly the hardware parts in contact with product, is minimized, especially with the implementation of an internal liner. With this internal liner, the packed bed can be separated from the column in intact form, or even prepared separately from, and outside of, the column.

主权项

1. A method of packing a chromatography column, said method comprising:
(a) placing a tube of flexible, water-impermeable material in a rigid column shell, said tube being open at a first end and closed at a second end with a bottom plate comprising a layer of porous material over a rigid and liquid-impermeable base, said layer of porous material facing said first end of said tube and said rigid and liquid-impermeable bottom plate base having a bottom plate port therein for discharge of liquid; (b) placing a slurry of separation medium particles in said tube within said rigid column shell and placing a piston in said tube over said slurry, said piston having a layer of porous material facing said slurry and a piston port for supply of liquid over said layer of porous material; (c) exerting a downward circulation of liquid between the said piston and the said bottom plate to settle and compact the said slurry; and (d) lowering said piston over said slurry to compact said particles into a packed bed.