METHODS AND COMPOSITIONS FOR ARRAY-BASED COUNTING

摘要

Methods, kits, and systems are disclosed for array-based counting. The method generally comprises obtaining data pertaining to one or more probes on an array and conducting probe-specific analysis of the data to determine a count of one or more objects hybridized to the probe on the array. The probe-specific analysis may comprise modeling a behavior of one or more probes. The objects hybridized to the probe may be nucleic acids from a sample. The sample may comprise a total quantity of nucleic acids of less than one genome equivalent. The array may be a tiling array. The methods, kits, and systems may be used for quantifying samples comprising low quantities of nucleic acids. The methods, kits, and systems may be used to diagnose a disease or condition in a subject. The methods, kits, and systems may be used to diagnose a fetal disorder.

主权项

1. A method comprising:
a. hybridizing a plurality of nucleic acids from a sample to one or more probes on an array to produce one or more hybridized nucleic acids, wherein the plurality of nucleic acids comprise one or more molecular species; b. scanning the array to obtain a detection signal for the one or more probes; c. converting the detection signal to a digital signal; and d. determining a count of the one or more hybridized nucleic acids based on a count of the digital signal.