POINT OF CARE SICKLE CELL TEST

摘要

The disclosure provides methods, kits, and devices for determining an amount of hemoglobin S and/or an amount of total hemoglobin, and optionally, expressing the amount of hemoglobin S as a percentage. Devices of the disclosure may be used as point-of-care diagnostic systems accessible for use to a layperson, i.e., an individual with minimal or no medical training or expertise.

主权项

1. A method for determining an amount of hemoglobin-S, comprising:
releasing an amount of total hemoglobin from red blood cells in a first volume of blood collected from a subject; mixing the amount of total hemoglobin, or an aliquot thereof, and a testing composition comprising an agent that selectively precipitates hemoglobin S to form a hemoglobin S precipitate in a mixture; measuring an optical absorbance of the hemoglobin S precipitate in the mixture, or an aliquot thereof, and translating the value of the optical absorbance into an amount of hemoglobin S.