METHOD FOR IDENTIFYING THE SEQUENCE OF ONE OR MORE VARIANT NUCLEOTIDES IN A NUCLEIC ACID MOLECULE
摘要
The invention relates to methods for identifying the sequence of one or more variant nucleotides in a nucleic acid molecule. The method involves cleaving a double- stranded nucleic acid molecule containing a mismatch with a mismatch-specific endonuclease which cleaves on the 3' side of the mismatch, and preserving the integrity of the variant nucleotide by ligating a double -stranded linker with a 3 '-overhang to said variant nucleotide. Because the variant nucleotide is immediately adjacent to the linker, PCR and/or sequence-by-synthesis analysis can be readily carried out.