MICRO CHIRAL REGULATION CELLULOSE CHROMATOGRAPHY STATIONARY PHASE, PREPARATION METHOD AND USE THEREOF

摘要

The present invention discloses a cellulose derivative shown as formula (I), which is obtained as follows: the hydroxyl at position 6 of microcrystalline cellulose is protected with triphenylchloromethane, and then reacted with acyl chloride or isocyanate. After the protection of the hydroxyl at positions 2 and 3 of microcrystalline cellulose, triphenylmethyl is removed under acidic conditions to expose the hydroxyl at position 6. Finally, the hydroxyl at position 6 is chiral derivatized with amino acid acyl chloride or polypeptide acyl chloride, to obtain a micro chiral regulation cellulose derivative. The micro chiral regulation cellulose derivative thus obtained is coated onto the surface of a silica gel support, to form a chiral stationary phase, which is filled in a stainless steel column to form a chiral column for the separation of various different types of chiral compounds. The preparation method of the present invention is not only efficient and convenient, but also safe and reliable. The chiral column thus formed has stable performance, high separation efficiency, and is suitable for large scale production.;

主权项

1. A cellulose derivative shown as formula (I): wherein, n=35˜350, R═CH3(CH2)mCO, m=0˜30, or Xa═—CH3, —Cl, —NO2, a=0˜5; Yb═C, N, O, S, b=0˜5; R* represents a chiral group, which is an N-protected amino acid acyl or N-protected polypeptide acyl.