发明名称 |
SOLUBLE GLYCOSAMINOGLYCANASES AND METHODS OF PREPARING AND USING SOLUBLE GLYCOSAMINOGLYCANASES |
摘要 |
<p>The invention relates to the discovery of novel soluble neutral active Hyaluronidase Glycoproteins (sHASEGPs), methods of manufacture, and their us e to facilitate administration of other molecules or to alleviate glycosaminoglycan associated pathologies. Minimally active polypeptide domai ns of the soluble, neutral active sHASEGP domains are described that include asparagine-linked sugar moieties required for a functional neutral active hyaluronidase domain. Included are modified amino- terminal leader peptides that enhance secretion of sHASEGP. The invention further comprises sialated and pegylated forms of a recombinant sHASEGP to enhance stability and serum pharmacokinetics over naturally occurring slaughterhouse enzymes. Further described are suitable formulations of a substantially purified recombinant sHASEGP glycoprotein derived from a eukaryotic cell that generate the proper glycosylation required for its optimal activity.</p> |
申请公布号 |
CA2598823(A1) |
申请公布日期 |
2006.08.31 |
申请号 |
CA20062598823 |
申请日期 |
2006.02.23 |
申请人 |
HALOZYME THERAPEUTICS, INC. |
发明人 |
DYLAN, TYLER M.;HALLER, MICHAEL F.;KELLER, GILBERT A.;FROST, GREGORY I.;KUNDU, ANIRBAN;BOOKBINDER, LOUIS H. |
分类号 |
C07K16/00;A01K67/027;A01K67/033;A01N37/18;A01N43/04;A61K38/17;A61K38/47;C07H21/02;C07H21/04;C07K1/00;C12N5/00;C12N9/24;C12N15/00;C12P21/06 |
主分类号 |
C07K16/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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