Method for detection of contaminants

摘要

A method and device for determining the presence and concentration of total microbial contamination or the presence and concentration of a specific microbial species is described. The method consists of a means of collection of a microbes form an air, liquid, surface or other sample and suspending them in fluid phase (3). An aliquot of the fluid phase (3) is introduced into a disposable test devise which allows filtration of the sample to concentrate the microbes and to remove extraneous substances including somatic cells, and concentration of the microbes. The total concentration of microbes is determined by adding a somatic and bacterial releasing reagent to a disposable test device (25) which composes a membrane (26) containing the luminescent reagents luciferin an luciferase, and introducing the disposable test devise (25) into a luminometer that can read the luminescence form the underside.