CREATININE BIOSENSOR

摘要

The invention relates to a method for producing biosensors that comprise at least two enzymes for the amperometric determination of enzymatically cleavable substances, such as creatinine, in biological liquids, the enzymes being immobilized on a working electrode. An enzyme is applied on the working electrode with one or more surface-active substances in an aqueous solution and is allowed to dry. The at least second enzyme is chemically immobilized thereon in a subsequent step, thereby allowing for shorter response times and stronger signals of the biosensor.