Microfluidic device for acoustic cell lysis

摘要

A microfluidic acoustic-based cell lysing device that can be integrated with on-chip nucleic acid extraction. Using a bulk acoustic wave (BAW) transducer array, acoustic waves can be coupled into microfluidic cartridges resulting in the lysis of cells contained therein by localized acoustic pressure. Cellular materials can then be extracted from the lysed cells. For example, nucleic acids can be extracted from the lysate using silica-based sol-gel filled microchannels, nucleic acid binding magnetic beads, or Nafion-coated electrodes. Integration of cell lysis and nucleic acid extraction on-chip enables a small, portable system that allows for rapid analysis in the field.

主权项

1. A microfluidic device for acoustic cell lysis, comprising:
a channel formed in a microfluidic substrate, adapted to flow a fluid comprising biological cells therein; and at least one acoustic transducer disposed on a lysis portion of the channel, adapted to propagate an acoustic wave in the fluid and thereby generate sufficient localized acoustic pressure in the lysis portion to lyse the biological cells in the fluid by acoustic pressure, wherein the operating frequency of the at least one acoustic transducer is between 10 and 100 MHz.