Method for the electro-addressable functionalization of electrode arrays

摘要

A method for preparing an electrochemical biosensor uses bias-assisted assembly of unreactive -onium molecules on an electrode array followed by post-assembly electro-addressable conversion of the unreactive group to a chemical or biological recognition group. Electro-addressable functionalization of electrode arrays enables the multi-target electrochemical sensing of biological and chemical analytes.

主权项

1. A method for preparing an electrochemical biosensor, comprising:
(i) providing an electrode array comprising a plurality of conducting or semiconducting electrodes; (ii) grafting a phenyl boronic acid diazonium salt having an unreactive group to the plurality of electrodes to form an assembly comprising a phenyl molecule comprising an unreactive group, wherein step (ii) comprises bias-assisted electrodeposition of the phenyl boronic acid diazonium salt to the plurality of conducting or semiconducting electrodes and wherein the bias-assisted electrodeposition comprises use of a first bias voltage; and (iii) applying a second bias voltage to at least one electrode of the plurality of conducting or semiconducting electrodes to convert the unreactive group of the phenyl molecule to a reactive group; wherein the reactive group comprises a chemical or biological recognition group that has selectivity for a target analyte.