| 摘要 |
Methods comprising the use of photoactivated chemical bleaching for detecting multiple targets in a biological sample are provided. The methods include the steps of providing a biological sample containing multiple targets, binding at least one probe to one or more target present in the sample, and observing a signal from the probe. The method further includes the steps of contacting the sample comprising the bound probe with a cationic or zwitterionic borate compound and irradiating the sample, thereby initiating a photoreaction that substantially inactivates the probe by photoactivated chemical bleaching. The method further includes the steps of binding at least one probe to one or more target present in the sample, and observing a signal from the probe. The process of binding, observing and bleaching may be iteratively repeated. |
| 主权项 |
1. A method of probing multiple targets in a biological sample comprising:
(a) providing a biological sample containing multiple targets; (b) binding at least one probe to one or more targets present in the sample; (c) observing a signal from the at least one probe bound in step (b); (d) contacting the sample comprising the bound probe of step (b) with a borate compound of Formula I;
R1R2R3R4BXa Iwhere each R1, R2, and R3 and R4 is, independently, an alkyl, an alkylaryl, an arylalkyl, or an aryl, with the proviso that at least one of R1, R2, and R3 and R4 is an alkyl group and at least one of R1, R2, and R3 and R4 group is positively charged;X is an anion; anda equals 0, 1, 2, or 3; (e) irradiating the sample of step (d); and (f) analyzing the sample for one or more targets. |
