发明名称 |
METHODS FOR PRODUCING SOLUBLE MULTI-MEMBRANE-SPANNING PROTEINS |
摘要 |
<p>Methods for producing membrane-spanning polypeptides in high yields, with native conformation, and/or in soluble form include solubilizing in non-ionic or zwitterionic detergents as well as use of promoters and expression vectors for expressing high yields of membrane-spanning polypeptides in bacterial cells. Mutated promoters provide tight control of membrane-spanning polypeptides in bacterial cell hosts.</p> |
申请公布号 |
EP1833970(A2) |
申请公布日期 |
2007.09.19 |
申请号 |
EP20050856114 |
申请日期 |
2005.12.22 |
申请人 |
GENENTECH, INC. |
发明人 |
ERNST, JAMES, A.;YANSURA, DANIEL, G.;KIM, HOK, SEON |
分类号 |
C12N15/70;C07K14/705;C07K16/18;G01N33/53;G01N33/68 |
主分类号 |
C12N15/70 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|